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Pichia Pastoris Host Cell DNA Residue Detection Kit

Pichia Pastoris Host Cell DNA Residue Detection Kit
Total

Product Summary

Alias:毕赤酵母PP宿主细胞 DNA 残留检测试剂盒

NO.:PP-HCD

LOQ:10fg/μL

Validity:12 months

Storage:-20℃

    Number:

Details

Quantitative analysis of DNA residue in recombinant protein expressed products, purified intermediate and finished products from host cell..

 

**1.Introduction**

 

This kit adopts Taqman probe fluorescence qPCR method. The kit has the advantages of high specificity and sensitivity by using specific primers & probes, LOQ can reach 10fg/μL level. This kit can be used in combination with our Magnetic Residual DNA Sample Preparation Kit (Cat#CG-DP100). The preparation process of DNA Control is completely consistent with that of other host cell DNA national standards, therefore it has high purity and no protein and ion interference to ensure the accuracy of the sample quantitative detection.

 

The kit provides DNA Dilution Buffer, which enables good replicate parallelism in a single experiment and good reproducibility between multiple experiments.

 

 **2. MATERIALS**

 

DNA Amplification

Components NO.

Components Name

Size

50T

100T

B1

2XqPCR Mix

0.625mL

1.25mL

B2

Primer&Probe Mix

100μL

200μL

B3

DNA Dilution Buffer

2×1.5mL

4×1.5mL

B4

DNA Control (10ng/μL)

25μL

50μL

B5

RNase-Free H2O

0.5mL

1mL

B6

50X ROX Reference Dye(Optional)

0.15ml

0.3ml

 

 

 **3.Shipping and Storage**
  • All components are shipped on dry ice or ice bags.
  • The kit should be stored at -20℃ and it is recommended to be used within one year. B2 should be stored protected from light.
  • B2/B3/B4 can be stored at -20℃ for 2 years, while B1/B5/B6 can be stored at -20℃ for 1 year. B1/B5/B6 can also be purchased together as a separate set.

 

 

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