Drug Names
Generic Name:Canine rabies virus antibody (RV-Ab) ELISA Kit
Purpose
Our Canine rabies virus antibody(RV-Ab) ELISA kit is to assay RV-Ab levels in Canine
serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided
in this kit has been pre-coated with an antigen specific to RV-Ab. Standards or samples are
added to the appropriate Microelisa stripplate wells and combined to the specific antigen.
Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for RV is added to each
Microelisa stripplate well and incubated. Free components are washed away. The TMB
substrate solution is added to each well. Only those wells that contain RV-Ab and HRP
conjugated RV antigen will appear blue in color and then turn yellow after the addition of the
stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of
450 nm. The presence of RV-Ab is determined by comparing with the CUTOFF value.