Determination of Significance：

ATP (adenosine 5'-triphosphate) is found broadly in animals, plants, microorganisms and cultured cells, which is described as the energy currency in all living systems. Detecting the content of ATP and calculating the level of energy charge can reflect the state of energy metabolism.

Measurement Principle：

Hexokinase (HK) catalyzes the synthesis of glucose and ATP into 6-phosphate glucose. 6-phosphate glucose dehydrogenase further catalyzes the dehydrogenation of glucose 6-phosphate and NADP to form NADPH. NADPH has a characteristic absorption peak at 340 nm, and the absorbance ratio of NADPH is in direct proportion to contents of ATP.

Self Provided：

Spectrophotometer, water bath/constant temperature incubator, refrigerated centrifuge, transferpettor, 1 mL quartz cuvette, ice, mortar/ homogenizer, distilled water and chloroform.

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