Determination of Significance：

Nitric Oxide Synthase (NOS, EC 1.14.13.39) is a kind of enzyme that catalyzes the synthesis of NO from L-arginine in vivo. It mainly exists in vascular smooth muscle cells, macrophages, endothelial cells, nerve cells, liver cells, glomerular membrane cells and other cells. As a cell signal molecule, it plays a very important role in the nervous, immune and cardiovascular systems of the body.

Measurement Principle：

NOS catalyzes L-arginine, molecular oxygen and NADPH to form NO and NADP+. NO is easily oxidized to form NO^2- and NO^3- in aqueous solution. Under acidic conditions, NO^2- and diazonium sulfonamide produce diazo compounds. The compounds could further couple with naphthyl vinyl diamine. The product has a characteristic absorption peak at 550 nm and its absorbance value can be measured to calculate NOS activity.

Self Provided：

Spectrophotometer/Microplate reader, centrifuge, balance, transferpettor, mortar/homogenizer/cell ultrasonic crusher, micro glass cuvette/96 well plate, ice and distilled water.

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