Product Features

· The total nucleic acid in body fluids can be extracted within 20-40 minutes, and virus DNA or virus RNA can be separated simultaneously.

· Compared with the traditional boiling method to extract virus DNA, our method increases detection sensitivity by more than 10-50 times.

· Compared with the traditional Trizol method to extract virus RNA, our approach enhances detection sensitivity by over 5-10 times.

· The inclusion of Carrier RNA effectively boosts virus detection sensitivity, allowing for the stable detection of viruses at a concentration of 100 copies/ml in body fluids.

· Our method eliminates the need for phenol-chloroform extraction and isopropanol precipitation steps.

· The extracted nucleic acid is pure, containing no impurities or inhibitors, enabling amplification using up to 1/2 of the reaction system volume as a template.

Product Introduction

Body fluid samples first undergo proteinase K digestion to release viral nucleic acid. Subsequently, we utilize column extraction technology to enable the viral nucleic acid to tightly adsorb onto the extraction column. By washing the extraction column once with a washing solution, impurities and PCR inhibitors remaining on the column can be effectively removed.

The viral nucleic acid on the extraction column can be directly eluted with Buffer TE and then immediately used in various molecular biology experiments.

Experimental Parameters

• Sample: Applicable to body fluid samples from different sources (including plasma, serum, urine, CSF, and cell culture supernatant).
• Starting sample volume: 200 μl of body fluid sample

Experimental data

Curve 1: Amplification curve using HBV DNA extracted from serum via traditional boiling method as a template (5 μl of separated DNA added to a 50 μl amplification system).

Curve 2: Amplification curve using HBV DNA extracted from the same serum (200 μl) with the Simgen Viral Nucleic Acid Extraction Kit as a template (5 μl of purified DNA added to a 50 μl amplification system).

Curve 3: Increasing the amount of HBV DNA template extracted using the Simgen Viral Nucleic Acid Extraction Kit showed no significant inhibitory effect, but the CT value decreased (25 μl of purified DNA added to a 50 μl amplification system).