Summary:

Purpose

Our Quick Step Human Lyme-IgM ELISA Kit is to for the qualitative determination of Lyme-IgM in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific toLyme-IgM. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for Lyme-IgM is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Lyme-IgM and HRP conjugated Lyme  antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of Lyme-IgM is determined by comparing with the CUT OFF value.

Determine the result
Test effectiveness: the average value of positive control ≥1.00; The average value of negative control ≤0.10. 
The critical value (CUT OFF) calculation: critical value = the average value of   negative control + 0.15
Negative judgement:if the OD value<CUT OFF, the sample is Human Lyme-IgM negative.
Positive judgement: if the OD value ≥CUT OFF,the sample is Human Lyme-IgM positive.