Summary:

Purpose

Our Quick Step Human Beta2-Glycoprotein 1 Antibody IgA (Beta2-GP1Ab IgA)elisa Kit is to for the qualitative determination of Beta2-GP1Ab IgA in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific toBeta2-GP1-IgA. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for Beta2-GP1-IgA is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Beta2-GP1-IgA and HRP conjugated Beta2-GP1  antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of Beta2-GP1-IgA is determined by comparing with the CUT OFF value.

Determine the result
Test effectiveness: the average value of positive control ≥1.00; The average value of negative control ≤0.10. 
The critical value (CUT OFF) calculation: critical value = the average value of   negative control + 0.15
Negative judgement:if the OD value<CUT OFF, the sample is Human Beta2-GP1Ab IgA negative.
Positive judgement: if the OD value ≥CUT OFF,the sample is Human Beta2-GP1Ab IgA positive.