Summary:

Purpose

Our Quick Step Human islet cell antibody,ICA ELISA Kit is to for the qualitative determination of ICA/IC-Ab in Human serum, plasma, culture media or any biological fluid.

Principle

The ELISA is based on the the qualitative enzyme immunofor technique.The Microplate provided in this kit has been pre-coated with an antigen specific to ICA/IC-Ab, make it to solid-phase antigen.Samples are added to the Microplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for ICA/IC-Ab is added to each Microplate well and incubated,so the antigen-antibody-Enzyme labeled antigen complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain ICA/IC-Ab and HRP conjugated IC antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of ICA/IC-Ab is determined by comparing with the CUT OFF value.

Determine the result
Test effectiveness: the average value of positive control ≥1.00; The average value of negative control ≤0.10.
The critical value (CUT OFF) calculation: critical value = the average value of negative control + 0.15
Negative judgement:if the OD value<CUT OFF, the sample is Human ICA/IC-Ab negative.
Positive judgement: if the OD value ≥CUT OFF,the sample is Human ICA/IC-Ab positive.