Summary:

Purpose

Our Quick Step Rat Orexin ELISA kit is to assay Orexin levels in Rat serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Orexin. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for Orexin is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Orexin and HRP conjugated Orexin antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Orexin. You can calculate the concentration of Orexin in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS1166Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.