Summary:

Purpose

Our Quick Step Human B7-H4 ELISA Kit is to assay B7-H4 levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to B7-H4. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for B7-H4 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain B7-H4 and HRP conjugated B7-H4 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of B7-H4. You can calculate the concentration of B7-H4 in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QSD4003Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.