Summary:

Purpose

Our Quick Step Rat α1-Antitrypsin (α1-AT)elisa Kit is to assay α1-AT levels in Rat serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an Antibody specific to α1-AT. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific Antibody. Then a Horseradish Peroxidase (HRP)- conjugated Antibody specific for α1-AT is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain α1-AT and HRP conjugated α1-AT Antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of α1-AT. You can calculate the concentration of α1-AT in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS1676Ra is provided for demonstration only. A standard curve should be generated for each set of samples assayed.