Summary:

Purpose

Our Quick Step Sheep leptin ELISA kit is to assay leptin levels in Sheep serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to leptin. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for leptin is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain leptin and HRP conjugated leptin antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of leptin. You can calculate the concentration of leptin in the samples by comparing the OD of the samples to the standard curve.

Typical Data

The standard curve of QS00009Sp is provided for demonstration only. A standard curve should be generated for each set of samples assayed.