Suitable for the detection of genomic DNA target sequences and cDNA target sequences after RNA reverse transcription.

• This product accurately quantifies and detects target genes with good reproducibility and high reliability.

• The product utilizes a hot-start DNA polymerase with anti-Taq antibodies, combined with an optimal buffer for Real-Time PCR, which can greatly enhance the amplification efficiency of PCR.

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Product Overview

2×Probe qPCR Mix is a pre-mixed solution specifically designed for probe-based real-time fluorescent quantitative PCR, formulated at a double concentration. This product incorporates a hot-start DNA polymerase with anti-Taq antibodies, combined with an optimal buffer for Real-Time PCR, significantly enhancing the amplification efficiency of PCR and enabling highly sensitive Real-Time PCR amplification reactions. The product comes with a separately provided ROX dye, making it suitable for use with market-leading fluorescent quantitative PCR instruments such as Applied Biosystems, Bio-Rad, Eppendorf, Roche, and more. Ideal for rapid Real-Time PCR amplification reactions, this product produces excellent standard curves across a wide quantitative range, allowing for accurate quantification and detection of target genes with good reproducibility and high reliability.

Transportation and Storage
Shipped under low-temperature conditions and stored at -20℃, with a shelf life of over two years.

Experimental Example
The following figure shows the amplification results using Simgen 2×Probe qPCR Mix with pig-specific primers and probes, after diluting pig DNA 10x, 100x, 1000x, and 10000x.