Containing Fast Pfu DNA Polymerase, the optimized two-fold concentrated PCR premix boasts extremely high amplification efficiency and broad template adaptability.

With a fidelity 52 times higher than regular Taq enzyme and 6 times higher than Pfu enzyme, 2×Fast Pfu PCR Master Mix can amplify at a speed of 15 seconds per kilobase. The PCR products have blunt ends, which can be directly ligated into blunt-end cloning vectors, or they can be adenylated at the ends before ligation into TA cloning vectors.

Product Introduction

2×Fast Pfu PCR Master Mix is an optimized, two-fold concentrated PCR premix that boasts exceptional amplification efficiency and broad template compatibility, making it suitable for nearly all PCR reactions. It is user-friendly and requires only 0.5 times the volume of the PCR system. To use, simply add the appropriate amount of 2×Fast Pfu PCR Master Mix, primers, and template, then top up the volume with ddH2O.

Containing Fast Pfu DNA Polymerase, 2×Fast Pfu PCR Master Mix offers a fidelity that is 52 times higher than regular Taq enzyme and 6 times higher than Pfu enzyme. It achieves an amplification speed of 15 seconds per kilobase, and the resulting PCR products have blunt ends, allowing for direct ligation into blunt-end cloning vectors or adenylation at the ends for subsequent ligation into TA cloning vectors.

Storage and Transportation

The product has a shelf life of over two years when stored at -20℃. When stored at 2~8℃ and transported under low temperature conditions, the shelf life is 6 months.

Experimental Data