One Step ELISA Kit For Mouse Interleukin 4 (IL4)_One Step ELISA KIT_Sunlong Medical™_SUNLONG BIOTECH CO., LTD-杭州圣隆生物科技有限公司

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One Step ELISA Kit For Mouse Interleukin 4 (IL4)

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Product Summary

Alias:BSF1; BCGF1 ;B Cell Stimulatory Factor 1; Lymphocyte stimulatory factor 1; B Cell Growth Factor; Binetrakin; Pitrakinra

NO.:One-EK011M

Assay type:One step sandwich elisa

Assay range:31.25pg/mL-2000pg/mL

Validity:Two Years

Species:Mouse

Sensitivity:5.75 pg/mL

Assay Time:1.5 hours

Sample Volume:Serum or plasma: 20μL; DMEM cell culture supernatant: 50μL

Manual

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Details

SAMPLE TYPE	Serum, plasma, DMEM cell culture supernatant
SAMPLE VOLUME	Serum or plasma: 20μL; DMEM cell culture supernatant: 50μL
SENSITIVITY	5.75 pg/mL
RANGE	31.25 pg/mL - 2000 pg/mL
ASSAY TIME	1.5 h
RECOVERY	76 % - 123 %
AVERAGE RECOVERY	0.96
PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL4 monoclonal antibody IL4 freeze-dried standard IL4 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for IL4 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL4 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL4 present is bound by the immobilized antibody and detect antibody. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL4 bound by the immobilizedantibody. The color development is stopped and the intensity of the color is measured bymicroplate reader.

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