One Step ELISA Kit For Rat Interleukin 1 Beta (IL1b)_One Step ELISA KIT_Sunlong Medical™_SUNLONG BIOTECH CO., LTD-杭州圣隆生物科技有限公司

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One Step ELISA Kit For Rat Interleukin 1 Beta (IL1b)

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Product Summary

Alias:IL1-B; IL1-Beta; IL1F2; IL-1beta;; Interleukin-1 Family Member 2; Catabolin

NO.:One-EK009R

Assay type:One step sandwich elisa

Assay range:7.81 pg/mL - 500 pg/mL

Validity:Two Years

Species:Rat

Sensitivity:1.80 pg/mL

Assay Time:1.5 hours

Sample Volume:Serum or plasma: 20μL; DMEM cell culture supernatant: 50μL

Manual

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Details

SAMPLE TYPE	Serum, plasma, DMEM cell culture supernatant
SAMPLE VOLUME	Serum or plasma: 20μL; DMEM cell culture supernatant: 50μL
SENSITIVITY	1.80 pg/mL
RANGE	7.81 pg/mL - 500 pg/mL
ASSAY TIME	1.5 h
RECOVERY	81 % - 123 %
AVERAGE RECOVERY	0.99
PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL1b monoclonal antibody IL1b freeze-dried standard IL1b detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for IL1b has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL1b (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL1b present is bound by the immobilized antibody and detect antibody. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL1b bound by the immobilizedantibody. The color development is stopped and the intensity of the color is measured bymicroplate reader.

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