Details
SAMPLE TYPE | Serum, plasma, DMEM cell culture supernatant |
---|---|
SAMPLE VOLUME | Serum or plasma: 20μL; DMEM cell culture supernatant: 50μL |
SENSITIVITY | 9.20 pg/mL |
RANGE | 15.63 pg/mL - 1000 pg/mL |
ASSAY TIME | 1.5 h |
RECOVERY | 88% – 114% |
AVERAGE RECOVERY | 0.99 |
PLATFORM | ELISA |
PLATE | Detachable 96-well plate |
SIZE | 96T/48T |
STORAGE | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
DELIVERY | 4℃ blue ice transportation |
COMPONENTS | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TGF-β1 monoclonal antibody TGF-β1 freeze-dried standard TGF-β1 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
ASSAY PRINCIPLE | This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for TGF-β1 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for TGF-β1 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then TGF-β1 present is bound by the immobilized antibody and detect antibody. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of TGF-β1 bound by the immobilizedantibody. The color development is stopped and the intensity of the color is measured bymicroplate reader. |
Partial purchase records (2)
Username | Quantity | bought time |
Fi*** | 2 | 2024-08-13 |
Um*** | 3 | 2024-03-11 |
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