Details
| SAMPLE TYPE | Serum, plasma, DMEM cell culture supernatant |
|---|---|
| SAMPLE VOLUME | Serum or plasma: 20μL; DMEM cell culture supernatant: 50μL |
| SENSITIVITY | 0.35 pg/mL |
| RANGE | 3.125 pg/mL – 200 pg/mL |
| ASSAY TIME | 1.5 h |
| RECOVERY | 88% – 114% |
| AVERAGE RECOVERY | 0.99 |
| INTRA PRECISION | 3.4% – 4.6% |
| INTER-PRECISION | 3.1% – 5.4% |
| PLATFORM | ELISA |
| PLATE | Detachable 96-well plate |
| SIZE | 96T/48T |
| STORAGE | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| DELIVERY | 4℃ blue ice transportation |
| COMPONENTS | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL18 monoclonal antibody Human IL18 freeze-dried standard IL18 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| ASSAY PRINCIPLE | This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilizedantibody. The color development is stopped and the intensity of the color is measured bymicroplate reader. |
Partial purchase records (22)
| Username | Quantity | bought time |
| Ga*** | 2 | 2024-09-06 |
| Ed*** | 2 | 2024-09-01 |
| Et*** | 1 | 2024-08-19 |
| Ca*** | 2 | 2024-08-04 |
| Vi*** | 1 | 2024-08-04 |
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