One Step ELISA Kit For Human Interleukin 6 (IL6)_One Step ELISA KIT_Sunlong Medical™_SUNLONG BIOTECH CO., LTD-杭州圣隆生物科技有限公司

Home > Sunlong Medical™ > One Step ELISA KIT > One Step ELISA Kit For Human Interleukin 6 (IL6)

One Step ELISA Kit For Human Interleukin 6 (IL6)

Total

Product Summary

Alias:IL-6,MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor

Save more [Favourable] 20% Discount for new kits

NO.:One-EK005H

Validity:Two Years

Assay type:One step sandwich elisa

Species:Human

Assay range:1.56 pg/mL – 100 pg/mL

Sensitivity:1.05 pg/mL

Sample Volume:Serum or plasma: 20μL; DMEM cell culture supernatant: 100μL

Assay Time:1.5 hours

NCBI_Gene:3569

UniProtKB: P05231

Manual

Product Spec:

48T

96T

Number:

Details

SAMPLE TYPE	Serum, plasma, DMEM cell culture supernatant
SAMPLE VOLUME	Serum or plasma: 20μL; DMEM cell culture supernatant: 100μL
SENSITIVITY	1.05 pg/mL
RANGE	1.56 pg/mL – 100 pg/mL
ASSAY TIME	1.5 h
RECOVERY	76% – 125%
AVERAGE RECOVERY	1.00
INTRA PRECISION	7.1%-9.5%
INTER-PRECISION	2.8% – 9.2%
PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-6 monoclonal antibody Human IL-6 freeze-dried standard IL-6 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human IL-6 has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for IL-6 (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-6 present is bound by the immobilized antibody and detect antibody, of which the latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-6 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader.

Partial purchase records (1)

Username	Quantity	bought time
Vi***	2	2024-03-07