Details
| SAMPLE TYPE | Serum, plasma, DMEM cell culture supernatant |
|---|---|
| SAMPLE VOLUME | Serum or plasma: 20μL; DMEM cell culture supernatant: 100μL |
| SENSITIVITY | 1.05 pg/mL |
| RANGE | 1.56 pg/mL – 100 pg/mL |
| ASSAY TIME | 1.5 h |
| RECOVERY | 76% – 125% |
| AVERAGE RECOVERY | 1.00 |
| INTRA PRECISION | 7.1%-9.5% |
| INTER-PRECISION | 2.8% – 9.2% |
| PLATFORM | ELISA |
| PLATE | Detachable 96-well plate |
| SIZE | 96T/48T |
| STORAGE | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| DELIVERY | 4℃ blue ice transportation |
| COMPONENTS | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-6 monoclonal antibody Human IL-6 freeze-dried standard IL-6 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| ASSAY PRINCIPLE | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human IL-6 has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for IL-6 (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-6 present is bound by the immobilized antibody and detect antibody, of which the latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-6 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader. |
Partial purchase records (1)
| Username | Quantity | bought time |
| Vi*** | 2 | 2024-03-07 |
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