One Step ELISA Kit For Human Tumor Necrosis Factor Alpha (TNFa)_One Step ELISA KIT_Sunlong Medical™

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One Step ELISA Kit For Human Tumor Necrosis Factor Alpha (TNFa)

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Product Summary

Alias:DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2

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NO.:One-EK003H

Sample Volume:Serum or plasma: 20μL; DMEM cell culture supernatant: 100μL

Species:Human

Assay type:One step sandwich elisa

Validity:Two Years

Assay Time:1.5 hours

Assay range:3.13 pg/mL–200 pg/mL

Sensitivity:1.77 pg/mL

NCBI_Gene:7124

UniProtKB:P01375

Manual

Product Spec:

48T

96T

Number:

Details

SAMPLE TYPE	Serum, plasma, DMEM cell culture supernatant
SAMPLE VOLUME	Serum or plasma: 10μL; DMEM cell culture supernatant: 33μL
SENSITIVITY	1.77 pg/mL
RANGE	3.13 pg/mL – 200 pg/mL
ASSAY TIME	1.5 h
RECOVERY	100% – 122%
AVERAGE RECOVERY	1.08
INTRA PRECISION	4.2% – 5.4%
INTER-PRECISION	5.5% – 6.2%
PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TNF-α monoclonal antibody Human TNF-α freeze-dried standard TNF-α detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for human TNF-α has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for TNF-α (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted intothe wells, then TNF-α present is bound by the immobilized antibody and detect antibody, of whichthe latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solutionreacts with HRP and color develops in proportion to the amount of TNF-α bound by the immobilized antibody. The color development is stopped and the intensity of the color is measuredby microplate reader.

Partial purchase records (24)

Username	Quantity	bought time
Is***	2	2024-09-03
Ma***	2	2024-08-09
Ta***	2	2024-08-04
Ca***	3	2024-08-04
Di***	3	2024-07-29