Details
| SAMPLE TYPE |
Cell culture supernates, serum and plasma |
|---|---|
| SAMPLE VOLUME | Serum or plasma: 10μL; cell culture supernatant: 50μL |
| SENSITIVITY | 4.92 pg/mL |
| RANGE | 7.81 pg/mL – 500 pg/mL |
| ASSAY TIME | 1.5 h |
| RECOVERY | 89% – 112% |
| AVERAGE RECOVERY | 1.02 |
| INTRA PRECISION | 2.3% – 3.0% |
| INTER-PRECISION | 2.1% – 5.3% |
| PLATFORM | ELISA |
| PLATE | Detachable 96-well plate |
| SIZE | 96T/48T |
| STORAGE | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| DELIVERY | 4℃ blue ice transportation |
| COMPONENTS | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-6 monoclonal antibody Mouse IL-6 freeze-dried standard IL-6 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| ASSAY PRINCIPLE | This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for Mouse IL-6 has been immobilized onto microwells, and two pellets of the biotin-linked detect antibody specific for IL-6 (light yellow) and streptavidin-HRP (purple) are pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted intothe wells, then IL-6 present is bound by the immobilized antibody and detect antibody, of whichthe latter is conjugated with streptavidin-HRP in the incubation. After washing, substrate solutionreacts with HRP and color develops in proportion to the amount of IL-6 bound by the immobilizedantibody. The color development is stopped and the intensity of the color is measured bymicroplate reader. |
Partial purchase records (3)
| Username | Quantity | bought time |
| Ka*** | 3 | 2024-09-11 |
| Ga*** | 1 | 2024-06-20 |
| Iv*** | 1 | 2024-03-10 |
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