Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

Kit features:

1, Specific detection of Waddlia chondrophila, no cross-reaction with other biological genomes.

2. Using hot-start Taq enzyme, you can Inhibit non-specific amplification and reduce background fluorescence.

3, with positive control sample, can be used Check the effectiveness of the kit.

4, with ROX reference dye, help Correct for loading errors and tube-to-tube differences.

5, with UDG enzyme and dUTP, can Reduce residual DNA contamination.

Chlamydia abortus, also known as Chlamydia abortus/Chlamydophila abortus, or Chlamydia abortus, is an obligate intracellular parasitic prokaryote, Gram-negative, first discovered in 1950, and was once classified as psittacosis A subtype of Chlamydia psittaci, officially established as an independent species in 1999. Chlamydia abortus mainly accumulates in the placenta, causing miscarriage, premature birth or stillbirth. It is the most common abortion factor in goats and sheep. It can also cause long-term subclinical infection in non-pregnant sheep. In sheep with no history of exposure, Chlamydia abortus can cause 25-60% of ewes to miscarry. In sheep in epidemic areas, the abortion rate can be reduced to 1-15%, and mainly occurs in exotic sheep and primiparous sheep. . Chlamydia abortus can parasitize a variety of mammals. In addition to ruminants, there are also pigs, horses, rabbits, guinea pigs, mice, foxes, minks, raccoon dogs, etc. It can also infect non-native animals. Mammals . Chlamydia abortus has regional epidemics and has been found in many countries around the world, often causing significant economic losses. Infected animals may also infect humans, causing miscarriages and respiratory disorders, thus posing a threat to public health.

For the detection of Chlamydia abortus, conventional methods include: in vitro The disadvantages of isolation and culture, immunohistochemistry and serology methods are that they are time-consuming and have insufficient sensitivity and specificity. PCR is a method of enzymatically synthesizing specific DNA fragments in vitro. It has high sensitivity, strong specificity and fast detection speed, and can be completed in only two or three hours. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution. The quantitative PCR method is divided into two indicating methods: dye and probe. On the premise of ensuring primer specificity, the dye method can achieve the same detection effect at a lower cost.

This kit uses dye method quantitative PCR, targeting Primers were designed based on the 16S-23S rRNA gene interval sequence to specifically identify Chlamydia abortus, and were verified by BLAST to only cross-react with the Chlamydia buteonis genome. Chlamydia buteonis is a chlamydia isolated from the North American red-shouldered buzzard in recent years. Its genetic relationship is between Chlamydia abortus and Chlamydia psittaci. Considering the difference in hosts, it will not affect the detection of Chlamydia abortus in mammals. This kit detects 9 differentNo non-specific signals were found for chlamydia, as well as 20 bacteria and 4 viruses that may cause similar symptoms; among 3 chlamydia-positive sheep samples, 1 was found to be positive for chlamydia abortus. This kit is suitable for the detection and identification of Chlamydia abortus.

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

试剂盒特点：

1，   特异性检测哺乳动物中的流产衣原体。

2，   使用热启动的Taq酶，可抑制非特异性扩增，降低背景荧光。

3，   带有阳性对照样品，可用于检验试剂盒有效性。

4，   带有ROX参比染料，帮助校正加样误差和管间差异。

5，   带有UDG酶和dUTP，可降低残留DNA的污染。

流产衣原体（Chlamydia abortus），也称为流产嗜衣原体/流产亲衣原体（Chlamydophila abortus）或羊牛流产衣原体，是一种专性细胞内寄生的原核生物，革兰氏阴性，1950年首次发现，曾被归类为鹦鹉热衣原体（Chlamydia psittaci）的一个亚型，于1999年正式确立为独立物种。流产衣原体主要聚集在胎盘，引起流产、早产或死产，是山羊和绵羊中最常见的流产因素，在非孕期羊中也会造成长期的亚临床感染。在无暴露史的羊群中，流产衣原体可引起25-60%的母羊流产，在疫区羊群中，流产率则可降到1-15%，并且主要发生在外来羊和初产羊。流产衣原体可寄生于多种哺乳动物，除了反刍动物（如：山羊、绵羊、牛和鹿）以外，还有猪、马、兔、豚鼠、小鼠、狐、貂、貉等，也可感染非哺乳动物（如：鸟类）。流产衣原体有地域流行性，全球许多国家都有发现，常造成重大经济损失。受感染的动物还可能感染人类，造成流产和呼吸功能紊乱，因此也会对公共健康造成威胁。

流产衣原体的检测，常规方法有：体外分离培养、免疫组化以及血清学方法等，缺点在于耗时，灵敏度和特异性都有所不足。PCR是一种体外酶促合成特异性DNA 片段的方法,灵敏度高，特异性强，检测速度快，只需两三个小时即可完成。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。定量PCR法又分为染料和探针两种指示方式，在保证引物特异性的前提下，染料法可以用更低的成本达到同样的检测效果。

本试剂盒采用染料法定量PCR，针对16S-23S rRNA基因区间序列设计引物，特异性识别流产衣原体，经BLAST验证，仅与Chlamydia buteonis基因组有交叉反应。Chlamydia buteonis是近年从北美赤肩鵟分离出来的衣原体，亲缘关系处于流产衣原体和鹦鹉热衣原体之间，考虑到宿主的差异，不会影响到哺乳动物流产衣原体的检测。本试剂盒检测了9种不同的衣原体，和可能引起类似症状的20种细菌和4种病毒，未发现非特异性信号；在3个衣原体阳性绵羊样本中，发现1个流产衣原体阳性。本试剂盒适用于流产衣原体的检测和鉴定。