Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

Kit features:

1, Specific detection of Waddlia chondrophila, no cross-reaction with other biological genomes.

2, High sensitivity, the lowest detection limit is lower than 10 copies/reaction.

3. DNA polymerase adopts hot start method. It can inhibit non-specific amplification and reduce background fluorescence.

4, with positive control sample, can be used Check the effectiveness of the kit.

5, with UDG enzyme and dUTP, can Reduce residual DNA contamination.

Waddlia chondrophilabelongs toThe Department of Chlamydia is a specialized Sexual intracellular parasitic bacteria were first isolated from aborted fetal cattle in 1990 and are considered to be pathogenic bacteria causing abortion in ruminants. In humans, Waddlia chondrophila serum antibody positivity is strongly associated with miscarriage and is therefore considered a possible causative factor. It can also be detected in respiratory samples from patients with bronchiolitis or pneumonia. to its existence. Studies have shown that Waddlia chondrophila can rapidly proliferate in human macrophages, causing cell lesions, causing macrophage lysis, and thus damaging the immune system.

Waddlia chondrophila cannot be cultured using traditional methods For detection, only PCR method can be used. The PCR method has the advantages of high sensitivity, strong specificity, and fast detection speed, which can be completed in only two to three hours, which can effectively make up for the shortcomings of the above two methods. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution.

This kit designs primers and probes for the 16S rRNA gene, which can specifically The identification of Waddlia chondrophila was verified by BLAST and showed no cross-reactivity with other biological genomes. This kit was used to detect 20 species of bacteria that were closely related or distributed, and no cross-reaction occurred. Nasopharyngeal aspirates from 32 patients with non-viral bronchiolitis were tested and 3 positive samples were obtained. It can be seen that this kit is suitable for the detection and identification of Waddlia chondrophila.

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

试剂盒特点：

1，   特异性检测Waddlia chondrophila，与其他生物基因组无交叉反应。

2，   灵敏度高，最低检测极限低于10个拷贝/反应。

3，   DNA聚合酶采用热启动方式，可抑制非特异性扩增，降低背景荧光。

4，   带有阳性对照样品，可用于检验试剂盒有效性。

5，   带有UDG酶和dUTP，可降低残留DNA的污染。

Waddlia chondrophila属于衣原体目（Chlamydiales order ）的华诊体科（或称石德菌科，Waddliaceae，WADDL为“华盛顿动物病诊断实验室”缩写），为专性细胞内寄生菌，最初是在1990年从流产胎牛中分离出来，被认为是引起反刍动物流产的致病菌。在人类当中，Waddlia chondrophila血清抗体阳性与流产有强关联性，因此也被认为是一个可能的致病因素，另外在毛细支气管炎或肺炎患者的呼吸道样本中也可检测到它的存在。有研究显示，Waddlia chondrophila可在人巨噬细胞中快速增殖，导致细胞产生病变，引起巨噬细胞裂解，进而损害免疫系统。

Waddlia chondrophila不能采用传统的培养法进行检测，因此只能采用PCR法。PCR法具有灵敏度高、特异性强的优点，检测速度快，只需两三个小时即可完成，可以有效弥补上述两种方法的不足。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。

本试剂盒针对16S rRNA基因设计引物和探针，可以特异性识别Waddlia chondrophila，经BLAST验证，与其他生物基因组没有交叉反应。使用本试剂盒检测了亲缘或分布较为接近的20种细菌，无交叉反应出现。对32个非病毒性毛细支气管炎患者的鼻咽吸出物进行检测，得到3个阳性样本。可见本试剂盒适用于Waddlia chondrophila的检测和鉴定。