Sunlong Medical™ Mouse IL-2 High Sensitivity ELISA Kit_High Sensitivity ELISA Kit_Sunlong Medical™

Home > Sunlong Medical™ > High Sensitivity ELISA Kit > Sunlong Medical™ Mouse IL-2 High Sensitivity ELISA Kit

Sunlong Medical™ Mouse IL-2 High Sensitivity ELISA Kit

Total

Product Summary

Alias:IL2 elisa kit | interleukin 2 elisa kit | IL-2 | Il-2

Save more [Favourable] 20% Discount for new kits

NO.:HS-EL0113Mo

Species:Mouse

Assay range:0.23pg/mL-15pg/mL

Sensitivity:0.05 pg/mL

Sample Volume:20 μL

Assay type:Sandwich Method

Validity:Two Years

Manual

Product Spec:

48T

96T

Number:

Details

Cat NO.:	HS-EL0113Mo
Product:	Sunlong Medical™ Mouse IL-2 High Sensitivity ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum \| plasma \| cell culture supernatant and other biological samples
Spike Recovery Range:	82% - 98%
Mean Spike Recovery:	0.92
CV of Intra plate:	4.5% - 4.9%
CV of Inter plate:	4.4% - 8.9%
NCBI_Gene:	16183
UniProtKB:	P04351

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-2 monoclonal antibody Mouse IL-2 freeze-dried standard IL-2 detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-2 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-2 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-2 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).