Details
|
Cat NO.:
|
HS-EL0152Hu |
|---|---|
|
Product:
|
Sunlong Medical™ Human IL-21 High Sensitivity ELISA Kit |
|
Storage
|
4℃ (unopened) for two years |
|
Shipping Condition
|
4℃ |
|
Sample Type:
|
Serum | plasma | cell culture supernatant and other biological samples |
|
Spike Recovery Range:
|
90% - 116% |
|
Mean Spike Recovery:
|
1.07 |
|
CV of Intra plate:
|
4.1% - 5.0% |
|
CV of Inter plate:
|
2.6% - 4.2% |
|
NCBI_Gene:
|
59067 |
|
UniProtKB:
|
Q9HBE4 |
| PLATFORM | ELISA |
|---|---|
| PLATE | Detachable 96-well plate |
| SIZE | 96T/48T |
| STORAGE | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| DELIVERY | 4℃ blue ice transportation |
| COMPONENTS | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-21 monoclonal antibody Human IL-21 freeze-dried standard IL-21 detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) |
| ASSAY PRINCIPLE | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-21 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-21 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-21 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Citations
Partial purchase records (10)
| Username | Quantity | bought time |
| Um*** | 1 | 2024-09-11 |
| Ga*** | 3 | 2024-07-07 |
| Um*** | 1 | 2024-07-03 |
| Um*** | 2 | 2024-05-22 |
| Na*** | 1 | 2024-05-15 |
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