Details
|
Cat NO.:
|
HS-EL0035Ra |
|---|---|
|
Product:
|
Sunlong Medical™ Rat IL-4 High Sensitivity ELISA Kit |
|
Storage
|
4℃ (unopened) for two years |
|
Shipping Condition
|
4℃ |
|
Sample Type:
|
Serum | plasma | cell culture supernatant and other biological samples |
|
Spike Recovery Range:
|
81% - 101% |
|
Mean Spike Recovery:
|
90% |
|
CV of Intra plate:
|
5.0 % - 6.8 % |
|
CV of Inter plate:
|
2.7% - 7.5 % |
|
NCBI_Gene:
|
287287 |
|
UniProtKB:
|
A0A0G2JVY1 |
| PLATFORM | ELISA |
|---|---|
| PLATE | Detachable 96-well plate |
| SIZE | 96T/48T |
| STORAGE | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| DELIVERY | 4℃ blue ice transportation |
| COMPONENTS | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-4 monoclonal antibody Rat IL-4 freeze-dried standard IL-4 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| ASSAY PRINCIPLE | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat IL-4 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-4 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-4 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Citations
Partial purchase records (23)
| Username | Quantity | bought time |
| He*** | 2 | 2024-09-11 |
| Um*** | 2 | 2024-09-10 |
| Um*** | 1 | 2024-09-04 |
| Di*** | 1 | 2024-08-30 |
| Sa*** | 3 | 2024-08-20 |
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