Sunlong Medical™ Rat IL-1β High Sensitivity ELISA Kit_High Sensitivity ELISA Kit_Sunlong Medical™

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Sunlong Medical™ Rat IL-1β High Sensitivity ELISA Kit

Total

Product Summary

Alias:IL1B elisa kit | interleukin 1 beta elisa kit | IL-1 beta | IL-1F2 | interleukin-1 beta

NO.:HS-EL0039Ra

Species:Rat

Assay range:7.81pg/mL-500pg/mL

Sensitivity:0.71 pg/mL

Sample Volume:20 μL

Assay type:Sandwich Method

Validity:Two Years

Manual

Product Spec:

48T

96T

Number:

Details

Cat NO.:	HS-EL0039Ra
Product:	Sunlong Medical™ Rat IL-1β High Sensitivity ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum \| plasma \| cell culture supernatant and other biological samples
Spike Recovery Range:	89% - 127%
Mean Spike Recovery:	106%
CV of Intra plate:	3.8 % - 5.2 %
CV of Inter plate:	4.9 % - 5.6 %
NCBI_Gene:	24494
UniProtKB:	Q5BKB0

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-1β monoclonal antibody Rat IL-1β freeze-dried standard IL-1β detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat IL-1β antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-1β present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-1β in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Citations

1.FERMT1 knockdown attenuates inflammation induced by intracerebral hemorrhage in rats via NLRP3/NF-κB pathway

2.Lycopene ameliorates locomotor activity and urinary frequency induced by pelvic venous congestion in rats

3.Coptisine mitigates diabetic nephropathy via repressing the NRLP3 inflammasome

4.Mitochondrial transfer from bone mesenchymal stem cells protects against tendinopathy both in vitro and in vivo

5.Yangqing Chenfei formula alleviates silica-induced pulmonary inflammation in rats by inhibiting macrophage M1 polarization

6.Protective effecs of baicalin magnesium on non-alcoholic steatohepatitis rats are based on inhibiting NLRP3/Caspase-1/IL-1β signaling pathway

7.Hyperoside exerts protective effects against anticardiolipin antibody-induced recurrent pregnancy loss in vivo and in vitro

8.P2X7R Mediates the Synergistic Effect of ATP and MSU Crystals to Induce Acute Gouty Arthritis

Partial purchase records (12)

Username	Quantity	bought time
Wi***	2	2024-09-12
Na***	1	2024-09-01
Di***	3	2024-08-06
Vi***	1	2024-07-25
Ad***	3	2024-07-19