Sunlong Medical™ Human IL-4 High Sensitivity ELISA Kit_High Sensitivity ELISA Kit_Sunlong Medical™

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Sunlong Medical™ Human IL-4 High Sensitivity ELISA Kit

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Product Summary

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NO.:HS-EL0279Hu

Species:Human

Assay range:0.25pg/mL-16pg/mL

Sensitivity:0.03 pg/mL

Sample Volume:20 μL

Assay type:Sandwich Method

Validity:Two Years

Manual

Product Spec:

48T

96T

Number:

Details

Cat NO.:	HS-EL0279Hu
Product:	Sunlong Medical™ Human IL-4 High Sensitivity ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum \| plasma \| cell culture supernatant and other biological samples
Spike Recovery Range:	94% - 105%
Mean Spike Recovery:	0.98
CV of Intra plate:	4.2% - 4.8%
CV of Inter plate:	3.6% - 8.1%
NCBI_Gene:	3565
UniProtKB:	P05112

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-4 monoclonal antibody Human IL-4 freeze-dried standard IL-4 detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-4 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-4 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-6 in the samples.

Citations

1.CLDN1 silencing suppresses the proliferation and migration of airway smooth muscle cells by modulating MMP14

2.The Effect of an Extract of Sappanwood, Protosappanin A and Protosappanin B on Osteogenesis in Periodontitis

3.Characteristics of Serum Autoantibody Repertoire and Immune Subgroup Variation of Tuberculosis-Associated Obstructive Pulmonary Disease

4.CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages

5.CLDN1 silencing suppresses the proliferation and migration of airway smooth muscle cells by modulating MMP14

6.Modified Gegen Qinlian decoction ameliorated ulcerative colitis by attenuating inflammation and oxidative stress and enhancing intestinal barrier function in vivo and in vitro

7.MicroRNA-143-3p ameliorates collagen-induced arthritis by polarizing naive CD4+T cells into Treg cells

Partial purchase records (4)

Username	Quantity	bought time
We***	2	2024-08-26
Ma***	2	2024-08-07
Sa***	2	2024-04-27
Ol***	2	2024-04-20