Details
|
Cat NO.:
|
EL0018Ra |
|---|---|
|
Product:
|
Sunlong Medical™ Rat IgA ELISA Kit |
|
Storage
|
4℃ (unopened) for two years |
|
Shipping Condition
|
4℃ |
|
Sample Type:
|
Serum | plasma | cell culture supernatant and other biological samples |
|
Spike Recovery Range:
|
82% - 105% |
|
Mean Spike Recovery:
|
0.95 |
|
CV of Intra plate:
|
2.7 % - 7.9 % |
|
CV of Inter plate:
|
3.9 % - 8.6 % |
|
NCBI_Gene:
|
100913063 |
|
UniProtKB:
|
F1MAC3 |
| PLATFORM | ELISA |
|---|---|
| PLATE | Detachable 96-well plate |
| SIZE | 96T/48T |
| STORAGE | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| DELIVERY | 4℃ blue ice transportation |
| COMPONENTS | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IgA monoclonal antibody Rat IgA freeze-dried standard IgA detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| ASSAY PRINCIPLE | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) technique. Specific anti-rat IgA antibodies are pre-coated on a high-affinity ELISA plate. Standard samples and test samples are added to the wells of the ELISA plate. After incubation, the IgA present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, HRP-labeled detection antibodies are added and incubated. After washing to remove unbound detection antibodies, the TMB chromogenic substrate is added for color development while avoiding light. The intensity of the color reaction is directly proportional to the concentration of IgA in the sample. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Citations
Partial purchase records (5)
| Username | Quantity | bought time |
| Et*** | 3 | 2024-09-03 |
| Xa*** | 3 | 2024-06-29 |
| Vi*** | 3 | 2024-04-22 |
| Ra*** | 1 | 2024-03-26 |
| Mi*** | 1 | 2024-03-25 |
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