Sunlong Medical™ Mouse IgM ELISA Kit_ELISA KIT_Sunlong Medical™_SUNLONG BIOTECH CO., LTD-杭州圣隆生物科技有限公司

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Sunlong Medical™ Mouse IgM ELISA Kit

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Product Summary

Alias:Mouse Immunoglobulin M ELISA Kit

NO.:EL0029Mo

Species:Mouse

Assay range:0.2ng/mL-32ng/mL

Sensitivity:19.26 pg/mL

Sample Volume:10 μL

Assay type:Sandwich Method

Validity:Two Years

Manual

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Details

Cat NO.:	EL0029Mo
Product:	Sunlong Medical™ Mouse IgM ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum \| plasma \| cell culture supernatant and other biological samples
Spike Recovery Range:	91% - 107%
Mean Spike Recovery:	1.02
CV of Intra plate:	3.2 % - 4.3 %
CV of Inter plate:	2.9 % - 7.6 %
NCBI_Gene:	Inquiry
UniProtKB:	Inquiry

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IgM monoclonal antibody Mouse IgM freeze-dried standard IgM detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IgM antibodies are precoated on a high-affinity ELISA plate. Standard samples and test samples are added to the wells of the ELISA plate. After incubation, IgM present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, detection antibodies labeled with horseradish peroxidase (HRP) are added and incubated. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IgM in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

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