Sunlong Medical™ Rat IL-10 ELISA Kit_ELISA KIT_Sunlong Medical™_SUNLONG BIOTECH CO., LTD-杭州圣隆生物科技有限公司

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Sunlong Medical™ Rat IL-10 ELISA Kit

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NO.:EL0032Ra

Species:Rat

Assay range:62.5pg/mL-4000pg/mL

Sensitivity:0.48 pg/mL

Sample Volume:20 μL

Assay type:Sandwich Method

Validity:Two Years

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Details

Cat NO.:	EL0032Ra
Product:	Sunlong Medical™ Rat IL-10 ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum \| plasma \| cell culture supernatant and other biological samples
Spike Recovery Range:	86% - 108%
Mean Spike Recovery:	0.98
CV of Intra plate:	3.4 % - 5.3 %
CV of Inter plate:	4.5 % - 6.1 %
NCBI_Gene:	25325
UniProtKB:	A0A7R8C3K4

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-10 monoclonal antibody Rat IL-10 freeze-dried standard IL-10 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat IL-10 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-10 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-10 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).