Sunlong Medical™ Rat IL-4 ELISA Kit_ELISA KIT_Sunlong Medical™_SUNLONG BIOTECH CO., LTD-杭州圣隆生物科技有限公司

Home > Sunlong Medical™ > ELISA KIT > Sunlong Medical™ Rat IL-4 ELISA Kit

Sunlong Medical™ Rat IL-4 ELISA Kit

Total

Product Summary

Save more [Favourable] 20% Discount for new kits

NO.:EL0036Ra

Species:Rat

Assay range:15.63pg/mL-1000pg/mL

Sensitivity:0.26 pg/mL

Sample Volume:20 μL

Assay type:Sandwich Method

Validity:Two Years

Manual

Number:

Details

Cat NO.:	EL0036Ra
Product:	Sunlong Medical™ Rat IL-4 ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum \| plasma \| cell culture supernatant and other biological samples
Spike Recovery Range:	86% - 106%
Mean Spike Recovery:	0.99
CV of Intra plate:	2.1 % - 2.7 %
CV of Inter plate:	3.1 % - 4.3 %
NCBI_Gene:	287287
UniProtKB:	A0A0G2JVY1

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-4 monoclonal antibody Rat IL-4 freeze-dried standard IL-4 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat IL-4 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-4 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-4 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Citations

Partial purchase records (3)