Sunlong Medical™ Rat IL-1β ELISA Kit_ELISA KIT_Sunlong Medical™_SUNLONG BIOTECH CO., LTD-杭州圣隆生物科技有限公司

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Sunlong Medical™ Rat IL-1β ELISA Kit

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Product Summary

Alias:IL1B elisa kit | interleukin 1 beta elisa kit | IL-1 beta | IL-1F2 | interleukin-1 beta

NO.:EL0040Ra

Species:Rat

Assay range:31.25pg/mL-2000pg/mL

Sensitivity:1.42 pg/mL

Sample Volume:20 μL

Assay type:Sandwich Method

Validity:Two Years

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Details

Cat NO.:	EL0040Ra
Product:	Sunlong Medical™ Rat IL-1β ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum \| plasma \| cell culture supernatant and other biological samples
Spike Recovery Range:	84% - 110%
Mean Spike Recovery:	1.02
CV of Intra plate:	5.0 % - 5.1 %
CV of Inter plate:	5.5 % - 6.7 %
NCBI_Gene:	24494
UniProtKB:	Q5BKB0

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-1β monoclonal antibody Rat IL-1β freeze-dried standard IL-1β detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat IL-1β antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-1β present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-1β in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).