Sunlong Medical™ human Vitamin D Binding Protein (Vitamin D BP)elisa kit_ELISA KIT_Sunlong Medical™

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Sunlong Medical™ human Vitamin D Binding Protein (Vitamin D BP)elisa kit

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Product Summary

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NO.:EL0223Hu

Species:Human

Assay range:78.13pg/mL-5000pg/mL

Sensitivity:40.15 pg/mL

Sample Volume:10 μL

Assay type:Sandwich Method

Validity:Two Years

Manual

Product Spec:

48T

96T

Number:

Details

Cat NO.:	EL0223Hu
Product:	Sunlong Medical™ Human Vitamin D Binding protein, Vitamin D BP ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum \| plasma \| cell culture supernatant and other biological samples
Spike Recovery Range:	95% - 114%
Mean Spike Recovery:	1.05
CV of Intra plate:	4.4 % - 5.0 %
CV of Inter plate:	4.0 % - 7.5 %
NCBI_Gene:	2638
UniProtKB:	P02774

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Vitamin D BP monoclonal antibody Human Vitamin D BP freeze-dried standard Vitamin D BP detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Vitamin D BP antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Vitamin D BP present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Vitamin D BP in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Partial purchase records (9)

Username	Quantity	bought time
Ya***	1	2024-08-13
Ad***	2	2024-08-12
Sa***	1	2024-08-09
Qu***	3	2024-07-29
Ka***	2	2024-06-20