Sunlong Medical™ Rat IL-6 High Sensitivity ELISA Kit_High Sensitivity ELISA Kit_Sunlong Medical™

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Sunlong Medical™ Rat IL-6 High Sensitivity ELISA Kit

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Product Summary

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NO.:HS-EL0033Ra

Species:Rat

Assay range:7.81pg/mL-500pg/mL

Sensitivity:1.8 pg/mL

Sample Volume:20 μL

Assay type:Sandwich Method

Validity:Two Years

Manual

Product Spec:

48T

96T

Number:

Details

Cat NO.:	HS-EL0033Ra
Product:	Sunlong Medical™ Rat IL-6 High Sensitivity ELISA Kit
Storage	4℃ (unopened) for two years
Shipping Condition	4℃
Sample Type:	Serum \| plasma \| cell culture supernatant and other biological samples
Spike Recovery Range:	95% - 106%
Mean Spike Recovery:	1.02
CV of Intra plate:	6.4% - 9.1%
CV of Inter plate:	6.1% - 9.6%
NCBI_Gene:	24498
UniProtKB:	P20607

PLATFORM	ELISA
PLATE	Detachable 96-well plate
SIZE	96T/48T
STORAGE	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
DELIVERY	4℃ blue ice transportation
COMPONENTS	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-6 monoclonal antibody Rat IL-6 freeze-dried standard IL-6 detect Antibody HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
ASSAY PRINCIPLE	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat IL-6 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-6 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-6 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).