Source	Prokaryotic expression
Host	E.coli
Endotoxin Level	<1.0EU per 1µg (determined by the LAL method)
Subcellular Location	Secreted, Extracellular matrix
Predicted Molecular Mass	65.5kDa
Accurate Molecular Mass	65kDa(Analysis of differences refer to the manual)
Residues & Tags	Gln27~Ala349 with N-terminal His and GST Tag
Buffer Formulation	20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits	Freeze-dried powder
Purity	> 80%
Isoelectric Point	7.9
Applications	Positive Control; Immunogen; SDS-PAGE; WB.

 

SEQUENCE

 

USAGE

 

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

 

STORAGE

 

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

 

STABILITY

 

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

Image


Figure. SDS-PAGE