Details
| Product Name | Anti-DNA Polymerase delta, catalytic subunit/PE |
| Chinese Name | PE标记的DNA聚合酶δ催化亚单位/DNA pol δ cat抗体 |
| Alias | CDC2; CDC2 homolog; DNA directed DNA polymerase delta 1; DNA directed polymerase delta 1; DNA pol delta 1; DNA polymerase delta catalytic subunit; DNA polymerase subunit delta p125; DPOD1_HUMAN; POLD; POLD 1; POLD1; Polymerase (DNA directed) delta 1 catalytic subunit 125kDa; Polymerase DNA directed delta 1 catalytic subunit 125kD. |
| Research Area | Cell biology Epigenetics |
| Immunogen Species | Rabbit |
| Clonality | Polyclonal |
| React Species | Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Sheep, ) |
| Applications | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| Molecular weight | 124kDa |
| Form | Lyophilized or Liquid |
| Concentration | 1mg/ml |
| immunogen | KLH conjugated synthetic peptide derived from human DNA Polymerase delta, catalytic subunit |
| Lsotype | IgG |
| Purification | affinity purified by Protein A |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| Product Detail |
background: DNA replication, recombination and repair, all of which are necessary for genome stability, require the presence of exonucleases (1). In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches (2). Exonucleases involved in these processes include DNA polymerases, including DNA pol ∂ and é. DNA pol ∂ consists of two subunits, p125 which interacts directly with the sliding DNA clamp protein PCNA, and p50 (3,4). DNA pol ∂ can be regulated by cell cycle proteins (5). DNA pol é is a multiple subunit enzyme, the catalytic subunit of which is encoded by the POL2 gene (6,7). The exact reactions catalyzed by DNA pol ∂ and é on leading and lagging strands have not yet been elucidated. Function: Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex. Subunit: Heterotetramer composed of subunits of 125 kDa, 50 kDa, 66 kDa and 12 kDa. The 125 kDa subunit contains the polymerase active site and most likely the active site for the 3'-5' exonuclease activity. Interacts with WRNIP1. Interacts with POLD4 and PCNA. Subcellular Location: Nucleus. Similarity: Belongs to the DNA polymerase type-B family. Contains 1 CysA-type zinc finger. Database links: Entrez Gene: 5424 Human Entrez Gene: 18971 Mouse Omim: 174761 Human SwissProt: P28340 Human SwissProt: P52431 Mouse Unigene: 279413 Human Unigene: 16549 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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