human IgG Fc antibody_Secondary Antibody_Antibody_SUNLONG BIOTECH CO., LTD-杭州圣隆生物科技有限公司

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human IgG Fc antibody

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Product Summary

Alias:Immunoglobulin G Fc fragment; human IgG-Fc; human IgG Fc fragment.

NO.:SL0365R

Clonality:Polyclonal

Immunogen Species:Rabbit

React Species:Human

Applications:WB-,IHC-P,IHC-F,IF,ELISA-

concentration:1mg/ml

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Details

Product Name	human IgG Fc
Chinese Name	兔抗人IgG-Fc段抗体
Alias	Immunoglobulin G Fc fragment; human IgG-Fc; human IgG Fc fragment.
literatures	Specific References (1) \| SL0365R has been referenced in 1 publications. [IF=4.566] Su Yan. et al. The Circ-CYP24A1-miR-224-PRLR Axis Impairs Cell Proliferation and Apoptosis in Recurrent Miscarriage. Front Physiol. 2022 Mar;0:347 IHC ; Human. PubMed:35309064
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,
Applications	WB=1:1000-3000,IHC-P=1:100-500,IHC-F=1:500-2000,IF=1:500-2000,ELISA=1:1000-6000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Form	Liquid
Concentration	1mg/ml
immunogen	human IgG-Fc purified from human plasma.
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	1M PBS(pH7.4)
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Product Detail	The Fc region is the constant region on an immunoglobulin molecule, the area that is exactly the same on all antibodies. The Fc region is found on the heavy chains and is not involved in binding antigens. SWISS: N/A Gene ID: N/A
Product Picture	Sample: Lane 1: Human Serum Primary: Anti-human IgG Fc (SL0365R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: kDa Observed band size: 50 kDa Paraformaldehyde-fixed, paraffin embedded (human colon ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (human IgG Fc) Polyclonal Antibody, Unconjugated (SL0365R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (human IgG Fc) Polyclonal Antibody, Unconjugated (SL0365R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (human IgG Fc) Polyclonal Antibody, Unconjugated (SL0365R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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