Mouse Anti-human CD3/Cy3 antibody (BF00112)_Primary Antibody_Antibody_SUNLONG BIOTECH CO., LTD-杭州圣隆生物科技有限公司

Home > Antibody > Primary Antibody > Mouse Anti-human CD3/Cy3 antibody (BF00112)

Mouse Anti-human CD3/Cy3 antibody (BF00112)

Total

Product Summary

Alias:CD3 epsilon; CD3e; CD3e antigen epsilon polypeptide TiT3 complex; CD3E antigen epsilon polypeptide; CD3E antigen, epsilon subunit; CD3e molecule epsilon; CD3e molecule, epsilon CD3 TCR complex; CD3e molecule, epsilon CD3-TCR complex; CD3E_HUMAN; IMD

Save more [Favourable] 20% discount for elisa kit

NO.:BF00112

Clonality:Monoclonal

Immunogen Species:Mouse

React Species:(predicted: Human,)

Applications:Flow Cyt Flow-Cyt

concentration:1mg/ml

Manual

Product Spec:

25T

50T

100T

Number:

Details

Aliases:	ACTR2; Actin Related Protein 2; ARP2; ARP2 Actin Related Protein 2 Homolog; Actin-Related Protein 2; Actin-Like Protein 2; ARP2 (Actin-Related Protein 2, Yeast) Homolog; ARP2 Actin-Related Protein 2 Homolog (Yeast)
Background:	UniProt Entry: P61160; NCBI Gene Entry: 10097
Application Information	Molecular Weight: Predicted, 45 kDa, observed, 37 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB610 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Arp2
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Arp2 expression in C2C12 cells using Arp2 antibody (Cat#66172, 1:2,000). Green, isotype control; red, Arp2.

Immunocytochemical staining of C2C12 cells with Arp2 antibody (Cat#66172, 1:1,000). Nuclei were stained blue with DAPI; Arp2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Arp2 antibody (Cat#66172). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Arp2 antibody (Cat#66172, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-Arp2 antibody (Cat#66172). Arp2 expression in wild type (WT) and Arp2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Arp2 antibody (Cat#66172, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Partial purchase records (0)

No one bought this product